Open AccessA FACTOR PROMOTING THE INITIATION OF GLOBIN SYNTHESIS IN A RABBIT RETICULOCYTE CELL-FREE SYSTEM
Author(s) -
Joseph E. Fuhr,
Irving M. London,
Arthur I. Grayzel
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.63.1.129
Subject(s) - reticulocyte , globin , incubation , protein biosynthesis , cell free system , biochemistry , biology , chemistry , microbiology and biotechnology , messenger rna , hemoglobin , in vitro , gene
The synthesis of globin has been studied in a reticulocyte cell-free system in which the initiation of polypeptide chain synthesis can proceed for as long as ten minutes of incubation. With this system, we have found evidence for a soluble factor in the reticulocyte that stimulates protein synthesis and appears to act by promoting initiation.