Open AccessSYNTHESIS OF PHAGE-SPECIFIC α- AND β-GLUCOSYL TRANSFERASES DIRECTED BY T-EVEN DNA in vitro
Author(s) -
Lawrence M. Gold,
Manfred Schweiger
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.62.3.892
Subject(s) - dna , in vitro , escherichia coli , bacteriophage , biochemistry , biology , dna synthesis , enzyme , transferase , mutant , protein biosynthesis , microbiology and biotechnology , chemistry , gene
—Bacteriophage T4 DNA, when added to a ribonucleic acid- and protein-synthesizing system from uninfectedEscherichia coli , directed thein vitro synthesis of virus-specific glucosyl transferases.The T4-specific α- and β-glucosyl transferases are synthesizedin vivo early after infection, and function to glucosylate the hydroxymethylcytosine residues of phage DNA. Thein vitro glucosyl transferase synthesis was dependent upon transcription of T4 DNA, as well as upon protein synthesis. DNA from T4 mutants unable to induce glucosyl transerases failed to induce enzyme formationin vitro , although protein synthesis was unimpaired.