THE FACILE ISOLATION OF A STRUCTURAL PHOSPHOLIPOPROTEIN FROM Hydrogenomonas facilis AND Neurospora crassa | Zendy

Glenn D. Kuehn | Zendy; Bruce A. McFadden | Zendy; Roy A. Johanson | Zendy; John M. Hill | Zendy; L. K. Shumway | Zendy

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THE FACILE ISOLATION OF A STRUCTURAL PHOSPHOLIPOPROTEIN FROM Hydrogenomonas facilis AND Neurospora crassa

Author(s) -

Glenn D. Kuehn,

Bruce A. McFadden,

Roy A. Johanson,

John M. Hill,

L. K. Shumway

Publication year - 1969

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.62.2.407

Subject(s) - neurospora crassa , moiety , biochemistry , biology , isolation (microbiology) , neurospora , amino acid , polyacrylamide gel electrophoresis , microbiology and biotechnology , chemistry , stereochemistry , enzyme , gene , mutant

The paper describes a new and gentle procedure for isolating “structural” phospholipoproteins from organisms and the first such isolation from a procaryotic microbe,Hydrogenomonas facilis .The amino acid composition of its protein moiety resembles that of “structural protein” from other sources. Although disc gel electrophoresis has shown the protein to be heterogeneous, this is attributed to aggregation.

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