Open AccessTHE FACILE ISOLATION OF A STRUCTURAL PHOSPHOLIPOPROTEIN FROM Hydrogenomonas facilis AND Neurospora crassa
Author(s) -
Glenn D. Kuehn,
Bruce A. McFadden,
Roy A. Johanson,
John M. Hill,
L. K. Shumway
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.62.2.407
Subject(s) - neurospora crassa , moiety , biochemistry , biology , isolation (microbiology) , neurospora , amino acid , polyacrylamide gel electrophoresis , microbiology and biotechnology , chemistry , stereochemistry , enzyme , gene , mutant
The paper describes a new and gentle procedure for isolating “structural” phospholipoproteins from organisms and the first such isolation from a procaryotic microbe,Hydrogenomonas facilis .The amino acid composition of its protein moiety resembles that of “structural protein” from other sources. Although disc gel electrophoresis has shown the protein to be heterogeneous, this is attributed to aggregation.