Open AccessTEMPERATURE-SENSITIVE MUTATIONS IN Drosophila melanogaster , III. DOMINANT LETHALS AND SEMILETHALS ON CHROMOSOME 2
Author(s) -
David T Suzuki,
Douglas Procunier
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.62.2.369
Subject(s) - complementation , mutant , ethyl methanesulfonate , drosophila melanogaster , biology , genetics , chromosome , microbiology and biotechnology , gene
Dominant temperature-sensitive lethal and semilethal (DTS-L ) mutations induced by ethyl methanesulfonate have been recovered in chromosome 2 ofDrosophila melanogaster . The 19 (0.39% of the chromosomes screened) characterized had greater than 65 per cent viability at 22° ± 2°C and less than 5 per cent at 29° ± 0.5°C. Fifteen of theDTS-L 's map genetically as point mutants, one appears to be a synthetic lethal, and the remaining three yielded sterile females. Ten differentDTS mutants had a high survival in triploid females at 29°C. ElevenDTS-L 's were found to map in the same genetic region (dp-b ). All behaved as recessive lethals at 22°C and failed to survive at 22°C when combined with mutants3 and12 . Crosses of the 11 mutants in all possible pairwise combinations yielded a circular complementation map with a side branch. The effective lethal phases and temperature-sensitive periods during development were determined for 15 of the mutants.