ENZYMATIC CLEAVAGE AND REPAIR OF TRANSFORMING DNA | Zendy

P.J. Laipis | Zendy; Baldomero M. Olivera | Zendy; A. T. Ganesan | Zendy

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ENZYMATIC CLEAVAGE AND REPAIR OF TRANSFORMING DNA

Author(s) -

P.J. Laipis,

Baldomero M. Olivera,

A. T. Ganesan

Publication year - 1969

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.62.1.289

Subject(s) - dna , cleavage (geology) , enzyme , dna repair , biochemistry , polynucleotide , microbiology and biotechnology , biology , dna damage , single strand , nucleotidyltransferase , bacillus subtilis , genetics , chemistry , gene , bacteria , rna , paleontology , fracture (geology)

Single-strand nicks caused by DNase I are capable of inactivatingB. subtilis transforming DNA. Under suitable conditions, the polynucleotide joining enzyme fromE. coli and a similar DPN-requiring activity fromB. subtilis can completely repair this damage, restore biological activity, and increase the single-strand molecular weight. The rates of inactivation of a single genetic marker and of a four-marker linkage group suggest that a single-strand nick is inactivating even when far from the site of genetic damage.

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