Open AccessENZYMATIC CLEAVAGE AND REPAIR OF TRANSFORMING DNA
Author(s) -
Philip J. Laipis,
Baldomero M. Olivera,
A. T. Ganesan
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.62.1.289
Subject(s) - dna , cleavage (geology) , enzyme , dna repair , biochemistry , polynucleotide , microbiology and biotechnology , biology , dna damage , single strand , nucleotidyltransferase , bacillus subtilis , genetics , chemistry , gene , bacteria , rna , paleontology , fracture (geology)
Single-strand nicks caused by DNase I are capable of inactivatingB. subtilis transforming DNA. Under suitable conditions, the polynucleotide joining enzyme fromE. coli and a similar DPN-requiring activity fromB. subtilis can completely repair this damage, restore biological activity, and increase the single-strand molecular weight. The rates of inactivation of a single genetic marker and of a four-marker linkage group suggest that a single-strand nick is inactivating even when far from the site of genetic damage.