Open AccessDIFFERENCES IN LEUCYL-TRANSFER RNA'S AND SYNTHETASE IN SOYBEAN SEEDLINGS
Author(s) -
Marianne B. Anderson,
Joe H. Cherry
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.62.1.202
Subject(s) - hypocotyl , cotyledon , transfer rna , acylation , biology , biochemistry , rna , botany , gene , catalysis
Charged leucyl-transfer RNA (leucyl-tRNA) of soybean cotyledons can be fractionated into six radioactive peaks on a Freon column. Four leucyl-tRNA peaks are observed with the homologous hypocotyl system. Hypocotyl synthetase preparations only slightly acylate the other two leucyl-tRNA's (species 5 and 6). Further, leucyl-tRNA5 and 6 of the hypocotyl are present in small amounts in comparison to the cotyledon. Therefore, the rate-limiting quantities of synthetase and tRNA5 and 6 obtained from the hypocotyl prevent the detection of these two species of tRNA when a homologous hypocotyl system is used. To measure the relative amounts of hypocotyl tRNA's, the cotyledon synthetase preparation is employed. Preliminary results show that 6-benzyladenine increases the acylation of leucyl-tRNA5 and 6 and decreases acylation of tRNA1 in the hypocotyl. Synthetase preparations from cytokinin-treated plants are not significantly affected.