Open AccessTHE EFFECTS OF PROTEASES ON THE TROPOCOLLAGEN MACROMOLECULE AND ON ITS AGGREGATION PROPERTIES
Author(s) -
Alan J. Hodge,
John H. Highberger,
Gottfried G.J. Deffner,
Francis O. Schmitt
Publication year - 1960
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.46.2.197
Subject(s) - induced pluripotent stem cell , proteases , cell , stem cell , microbiology and biotechnology , cardiac electrophysiology , in vitro , myocyte , biophysics , drug discovery , chemistry , electrophysiology , biology , biochemistry , neuroscience , embryonic stem cell , enzyme , gene
density distribution has recently been directly demonstrated in electron micrographs of formaldehyde-fixed SIS-type aggregates by Kuhn, K., U. Hofmann, and W. Grassmann (Naturwiss., 46, 512 1959). I For this purpose the segments were stained by exposure to 0.002 M uranyl nitrate for 1 minute and washed with distilled water before drying on the grid. Under these conditions the uranium in solution is cationic (see Horne, R. A., C. D. Coryell, and L. S. Goldring, Ann. Rev. Nuclear Sci., 6, 163, 1956). 10 Kuhn, K., W. Grassmann, and U. Hofmann, Z. Naturforsch., 13, 154 (1958). 11 Nemetschek, T., W. Grassmann, and U. Hofmann, Z. Naturforsch., lOb, 61 (1955). ,2Kiihn, K., Das Leder, 9, 217 (1958). 13 Bear, R. S., and R. S. Morgan, in Connective Tissue, ed. R. E. Tunbridge (London: Blackwell, 1957), p. 321. 14 Schmitt, F. 0., J. 0. Gross, and J. H. Highberger, Exp. Cell Res., Suppl. 3, 326 (1955). 15 Tomlin, S. G., Proc. Internal. Wool Textile Res. Conf., Australia, B, 187 (1955).
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