Open AccessRecombinant hepatitis E virus genomes infectious for primates: Importance of capping and discovery of a cis-reactive element
Author(s) -
Suzanne U. Emerson,
M. Zhang,
XiangJin Meng,
Hanh Nguyen,
Marisa St. Claire,
Sugantha Govindarajan,
Yuan-Cheng Huang,
Robert H. Purcell
Publication year - 2001
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.251555098
Subject(s) - virology , biology , complementary dna , recombinant dna , capsid , clone (java method) , virus , infectivity , genome , rna , gene , microbiology and biotechnology , genetics
Hepatitis E virus recombinant genomes transcribed in vitro from two cDNA clones differing by two nucleotides were infectious for chimpanzees. However, one cDNA clone encoded a virus that was attenuated for chimpanzees and unable to infect rhesus monkeys. The second cDNA clone encoded a virus that infected both chimpanzees and rhesus monkeys and caused acute hepatitis in both. One mutation differentiating the two clones identified a cis-reactive element that appeared to overlap the 3' end of the capsid gene and part of the 3' noncoding region. Capping of the RNA transcripts was essential for infectivity.
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