“Kiss and run” exocytosis at hippocampal synapses | Zendy

Charles F. Stevens | Zendy; James H. Williams | Zendy

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“Kiss and run” exocytosis at hippocampal synapses

Author(s) -

Charles F. Stevens,

James H. Williams

Publication year - 2000

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.230438697

Subject(s) - exocytosis , kiss and run fusion , vesicle , neurotransmitter , synaptic vesicle , kiss (tnc) , hippocampal formation , electrophysiology , neuroscience , microbiology and biotechnology , glutamate receptor , chemistry , vesicle fusion , biophysics , biology , biochemistry , membrane , central nervous system , computer network , receptor , computer science

We have combined electrophysiology and imaging to measure the release of neurotransmitter and fluorescent dye at synapses of cultured hippocampal neurons. These experiments have revealed a "kiss and run" mode of exocytosis in which synaptic vesicles release glutamate normally but do not permit dye to enter or escape from the vesicle. During "kiss and run," the vesicle interior may be exposed very transiently (<6 ms), or a special configuration of the fusion pore may prevent dye exchange. We estimate that about 20% of the vesicles normally use this "kiss and run" pathway, and that the fraction of "kiss and run" events can be increased to over 80% by superfusing the synapses with hypertonic solution.

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