Virus-encoded suppressor of posttranscriptional gene silencing targets a maintenance step in the silencing pathway | Zendy

César Llave | Zendy; Kristin D. Kasschau | Zendy; James C. Carrington | Zendy

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Virus-encoded suppressor of posttranscriptional gene silencing targets a maintenance step in the silencing pathway

Author(s) -

César Llave,

Kristin D. Kasschau,

James C. Carrington

Publication year - 2000

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.230334397

Subject(s) - biology , gene silencing , transgene , dna methylation , methylation , gene , genetics , rna silencing , rna interference , agrobacterium tumefaciens , suppressor , small rna , microbiology and biotechnology , tobacco etch virus , rna , virus , gene expression , plant virus , potyvirus

Certain plant viruses encode suppressors of posttranscriptional gene silencing (PTGS), an adaptive antiviral defense response that limits virus replication and spread. The tobacco etch potyvirus protein, helper component-proteinase (HC-Pro), suppresses PTGS of silenced transgenes. The effect of HC-Pro on different steps of the silencing pathway was analyzed by using both transientAgrobacterium tumefaciens -based delivery and transgenic systems. HC-Pro inactivated PTGS in plants containing a preexisting silenced β-glucuronidase (GUS) transgene. PTGS in this system was associated with both small RNA molecules (21–26 nt) corresponding to the 3′ proximal region of the transcribed GUS sequence and cytosine methylation of specific sites near the 3′ end of the GUS transgene. Introduction of HC-Pro into these plants resulted in loss of PTGS, loss of small RNAs, and partial loss of methylation. These results suggest that HC-Pro targets a PTGS maintenance (as opposed to an initiation or signaling) component at a point that affects accumulation of small RNAs and methylation of genomic DNA.

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