The construction of an EST database for Bombyx mori and its application | Zendy

Kazuei Mita | Zendy; Mitsuoki Morimyo | Zendy; Kazuhiro Okano | Zendy; Yoshiko Koike | Zendy; Junko Nohata | Zendy; Hideki Kawasaki | Zendy; Keiko KadonoOkuda | Zendy; Kimiko Yamamoto | Zendy; Masataka G. Suzuki | Zendy; Toru Shimada | Zendy; Marian R. Goldsmith | Zendy; Susumu Maeda | Zendy

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The construction of an EST database for Bombyx mori and its application

Author(s) -

Kazuei Mita,

Mitsuoki Morimyo,

Kazuhiro Okano,

Yoshiko Koike,

Junko Nohata,

Hideki Kawasaki,

Keiko KadonoOkuda,

Kimiko Yamamoto,

Masataka G. Suzuki,

Toru Shimada,

Marian R. Goldsmith,

Susumu Maeda

Publication year - 2003

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.2234984100

Subject(s) - bombyx , biology , expressed sequence tag , cdna library , gene , bombyx mori , complementary dna , database , genetics , genome , housekeeping gene , genomic library , computational biology , gene expression , peptide sequence , computer science

To build a foundation for the complete genome analysis of Bombyx mori, we have constructed an EST database. Because gene expression patterns deeply depend on tissues as well as developmental stages, we analyzed many cDNA libraries prepared from various tissues and different developmental stages to cover the entire set of Bombyx genes. So far, the Bombyx EST database contains 35,000 ESTs from 36 cDNA libraries, which are grouped into approximately 11,000 nonredundant ESTs with the average length of 1.25 kb. The comparison with FlyBase suggests that the present EST database, SilkBase, covers >55% of all genes of Bombyx. The fraction of library-specific ESTs in each cDNA library indicates that we have not yet reached saturation, showing the validity of our strategy for constructing an EST database to cover all genes. To tackle the coming saturation problem, we have checked two methods, subtraction and normalization, to increase coverage and decrease the number of housekeeping genes, resulting in a 5-11% increase of library-specific ESTs. The identification of a number of genes and comprehensive cloning of gene families have already emerged from the SilkBase search. Direct links of SilkBase with FlyBase and WormBase provide ready identification of candidate Lepidoptera-specific genes.

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