An mRNA structure that controls gene expression by binding FMN | Zendy

Wade C. Winkler | Zendy; Smadar CohenChalamish | Zendy; Ronald R. Breaker | Zendy

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An mRNA structure that controls gene expression by binding FMN

Author(s) -

Wade C. Winkler,

Smadar CohenChalamish,

Ronald R. Breaker

Publication year - 2002

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.212628899

Subject(s) - riboswitch , flavin mononucleotide , operon , biology , ribosomal binding site , bacillus subtilis , gene , transcription (linguistics) , gene expression , regulation of gene expression , binding site , genetics , regulatory sequence , messenger rna , biochemistry , translation (biology) , flavin group , non coding rna , enzyme , linguistics , philosophy , escherichia coli , bacteria

The RFN element is a highly conserved domain that is found frequently in the 5'-untranslated regions of prokaryotic mRNAs that encode for flavin mononucleotide (FMN) biosynthesis and transport proteins. We report that this domain serves as the receptor for a metabolite-dependent riboswitch that directly binds FMN in the absence of proteins. Our results also indicate that in Bacillus subtilis, the riboswitch most likely controls gene expression by causing premature transcription termination of the ribDEAHT operon and precluding access to the ribosome-binding site of ypaA mRNA. Sequence and structural analyses indicate that the RFN element is a natural FMN-binding aptamer, the allosteric character of which is harnessed to control gene expression.

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