Open AccessThe origin of a developmentally regulatedIghreplicon is located near the border of regulatory domains forIghreplication and expression
Author(s) -
Jie Zhou,
Nasrin Ashouian,
Marc Délépine,
Fumihiko Matsuda,
Christophe Chevillard,
Roy Riblet,
Carl L. Schildkraut,
Barbara K. Birshtein
Publication year - 2002
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.212392399
Subject(s) - replicon , biology , gene , locus (genetics) , regulatory sequence , genetics , microbiology and biotechnology , regulation of gene expression , plasmid
The 3' Ig heavy chain locus (Igh) regulatory region is the most downstream known element of the murine Igh gene cluster. We report here that the nearest non-Igh genes-Crip, Crp2, and Mta1-are located approximately 70 kb further downstream and are beyond the end of the domain of Igh transcriptional regulation. We have localized an origin of replication in MEL cells to a 3-kb segment located between the 3' Igh regulatory region and Crip. Sequences downstream of this origin are replicated by forks that move in both directions. Sequences upstream of this origin (Igh-C, -D, and -J) are replicated in a single direction through a 500-kb segment in which no active bidirectional origins can be detected. We propose that this origin may lie at or near the end of the Igh regulation domain.