Open AccessMass cytometry reveals single-cell kinetics of cytotoxic lymphocyte evolution in CMV-infected renal transplant patients
Author(s) -
Kenichi Ishiyama,
Janice Arakawa-Hoyt,
Oscar A. Aguilar,
Izabella Damm,
Parhom Towfighi,
Tara K. Sigdel,
Stanley Tamaki,
Joël Babdor,
Matthew H. Spitzer,
Elaine F. Reed,
Minnie M. Sarwal,
Lewis L. Lanier,
AUTHOR_ID
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2116588119
Subject(s) - cytotoxic t cell , immunology , mass cytometry , cd8 , biology , immune system , transplantation , in vitro , medicine , phenotype , biochemistry , gene
Significance Memory-like NK cells (NKG2C+ CD57+ FcεRIγ– ) are established during CMV infection. Here, mass cytometry tracked the in vivo kinetics of CMV-induced memory NK cells generation and identified a unique subset of NKG2C+ CD57+ FcεRIγlow–dim as potentially prememory-like NK cells in CMV-infected kidney transplant patients. The study demonstrated that prememory-like NK cells with a high cytotoxic profile proliferate along with accumulation of new memory-like NK cells, whereas preexisting memory-like NK cells decreased in the peripheral blood after transplantation. Moreover, NKG2C+ CD8+ T cells and cytotoxic γδ T cells also expand during CMV infection. This interplay of three different cytotoxic lymphocytes demonstrates a combinatorial immune response against CMV infection, which may contribute to preventing CMV-associated complication in organ transplantation.