Open AccessMolecular characterization of Barrett’s esophagus at single-cell resolution
Author(s) -
Georg A. Busslinger,
Buys de Barbanson,
Rurika Oka,
Bas L. Weusten,
Michiel de Maat,
Richard van Hillegersberg,
Lodewijk A.A. Brosens,
Ruben van Boxtel,
Alexander van Oudenaarden,
Hans Clevers
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2113061118
Subject(s) - barrett's esophagus , esophagus , biology , biopsy , cell , pathology , single cell analysis , gene , adenocarcinoma , microbiology and biotechnology , cancer research , cancer , genetics , medicine , anatomy
Significance Barrett’s esophagus (BE), the premalignant condition of esophageal adenocarcinoma, is categorized into different stages which correlate with the risk of developing carcinoma. We performed single-cell DNA-sequencing experiments with fresh biopsies, which revealed the appearance of a specific T > C and T > G mutational signature, known as COSMIC signature SBS17, in BE cells that are chromosomally unstable. The SBS17-specific mutations were, however, not detected in chromosomally stable BE cells. Additionally, we performed single-cell RNA sequencing experiments which identified seven genes that facilitate the distinction between different BE stages on histological sections.