Open AccessCholesterol is required for transcriptional repression by BASP1
Author(s) -
Amy E. Loats,
Samantha Carrera,
Anna F Fleming,
Abigail R E Roberts,
Alice Sherrard,
Eneda Toska,
Alexander J. Moorhouse,
Kathryn F. Medler,
Stefan G. E. Roberts
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2101671118
Subject(s) - chromatin , corepressor , transcriptional regulation , biology , psychological repression , repressor , microbiology and biotechnology , transcription factor , histone , cholesterol , gene , coactivator , promoter , regulation of gene expression , genetics , gene expression , biochemistry
Significance Cholesterol is present within the cell nucleus, where it associates with chromatin, but to date, a direct role for cholesterol in nuclear processes has not been identified. We demonstrate that the transcriptional repressor brain acid soluble protein 1 (BASP1) directly interacts with cholesterol within the cell nucleus through a consensus cholesterol interaction motif. BASP1 recruits cholesterol to the promoter region of target genes, where it is required to mediate chromatin remodeling and transcriptional repression. Our work demonstrates that cholesterol plays a direct role in transcriptional regulation.