Open AccessReplication-dependent histone biosynthesis is coupled to cell-cycle commitment
Author(s) -
Claire Armstrong,
Sabrina L. Spencer
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2100178118
Subject(s) - histone , cell cycle , dna replication , biology , control of chromosome duplication , microbiology and biotechnology , dna synthesis , histone h2a , histone methylation , s phase , histone methyltransferase , eukaryotic dna replication , dna re replication , cell , genetics , dna , gene , gene expression , dna methylation
Significance Current thinking limits replication-dependent (RD) histone biosynthesis to S phase of the cell cycle, with histone production initiated concomitantly with DNA replication. This work examines the cell-cycle timing of RD histone biosynthesis in single human cells and shows that while there is indeed a burst of RD histone production in S phase, RD histone production actually begins earlier in G1, at the point of cell-cycle commitment. These results demonstrate that cells born committed to the subsequent cell cycle build up a small pool of histones before initiating DNA replication, thereby safeguarding against a loss of genome integrity, and are distinct from cells that have exited the cell cycle and do not require histone synthesis until cell-cycle reentry.