Open AccessProbing the binding specificities of human Siglecs by cell-based glycan arrays
Author(s) -
Christian Büll,
Rebecca Nason,
Lingbo Sun,
Julie Van Coillie,
Daniel Madriz Sørensen,
Sam J. Moons,
Zifeng Yang,
Steven Arbitman,
Steve M. Fernandes,
Shoei Furukawa,
Ryan McBride,
Corwin M. Nycholat,
Gosse J. Adema,
James C. Paulson,
Ronald L. Schnaar,
Thomas J. Boltje,
Henrik Clausen,
Yoshiki Narimatsu
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2026102118
Subject(s) - glycan , glycoconjugate , context (archaeology) , sialic acid , biology , siglec , computational biology , glycosylation , cell , galectin , cd22 , microbiology and biotechnology , biochemistry , glycoprotein , cd19 , paleontology
Significance Siglecs are immunomodulatory receptors that recognize sialic acid–carrying glycans with important functions in immunity. However, many of their natural ligands are poorly defined. We generated a cell-based glycan array comprised of a library of isogenic human cells displaying the greater complexity of sialic acids on diverse glycan structures and glycoconjugates in the natural context of the cell surface. We applied this array to reveal fine binding specificities of Siglecs for sialoglycans, informed of the underlying required glycosyltransferase genes, and provided evidence for selective binding context provided by glycan presentation on distinct protein sequences. Insight into the fine binding specificities of Siglecs will advance understanding their diverse biological functions and benefit therapeutic targeting in autoimmunity, inflammation, cancer, and Alzheimer’s disease.