Open AccessEndoplasmic reticulum membrane receptors of the GET pathway are conserved throughout eukaryotes
Author(s) -
Lisa Yasmin Asseck,
Dietmar Gerald Mehlhorn,
Jhon RiveraMonroy,
Martiniano María Ricardi,
Holger Breuninger,
Niklas Wallmeroth,
Kenneth Wayne Berendzen,
Minou Nowrousian,
Shuping Xing,
Blanche Schwappach,
Martin Bayer,
Christopher Grefen
Publication year - 2020
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2017636118
Subject(s) - endoplasmic reticulum , biology , arabidopsis , microbiology and biotechnology , protein targeting , membrane protein , chaperone (clinical) , conserved sequence , secretory pathway , gene , genetics , computational biology , peptide sequence , membrane , mutant , golgi apparatus , medicine , pathology
Significance The GET pathway is required for the insertion of tail-anchored (TA) membrane proteins in the endoplasmic reticulum (ER) of yeast and mammals. Some orthologous genes had also been identified in higher plants with the exception of one of the two ER membrane receptors required for membrane insertion. Get2/CAML is required for the pathway’s cytosolic chaperone to dock and release its TA protein cargo. Here we report the identification of the elusive plant GET pathway receptor through an interaction screen inArabidopsis . The candidate allows detection of further Get2/CAML orthologs in higher plants, revealing conservation and function of structural features across kingdoms. Additionally, our results demonstrate that these features, rather than sequence conservation, determine functionality of the candidate within the pathway.