CtIP-mediated DNA resection is dispensable for IgH class switch recombination by alternative end-joining | Zendy

Xiaobin S. Wang | Zendy; Junfei Zhao | Zendy; Foon Wu-Baer | Zendy; Zhengping Shao | Zendy; Brian J. Lee | Zendy; Olivia M. Cupo | Zendy; Raúl Rabadán | Zendy; Jean Gautier | Zendy; Richard Baer | Zendy; Shan Zha | Zendy

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CtIP-mediated DNA resection is dispensable for IgH class switch recombination by alternative end-joining

Author(s) -

Xiaobin S. Wang,

Junfei Zhao,

Foon Wu-Baer,

Zhengping Shao,

Brian J. Lee,

Olivia M. Cupo,

Raúl Rabadán,

Jean Gautier,

Richard Baer,

Shan Zha

Publication year - 2020

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.2010972117

Subject(s) - non homologous end joining , endonuclease , recombination , dna , v(d)j recombination , biology , immunoglobulin class switching , microbiology and biotechnology , dna repair , genetics , gene , antibody , b cell

Significance Ig CSR occurs through the nonhomologous end-joining pathway. In NHEJ-deficient cells, substantial CSR can be achieved through the Alt-EJ pathway. High-throughput analyses of IgH CSR junctions in NHEJ-deficient cells show that in contrast to endonuclease-generated breaks, Alt-EJ-mediated CSR can occur without CtIP-mediated DNA resection. The first temporal analyses of CSR junctions in NHEJ-proficient cells further showed that early Sμ internal deletion junctions are enriched for MH. Taken together, the results identified repetitive core IgH switch regions as favorable substrates for MH-mediated recombination independent of resection.

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