Open AccessThe AMPK-PP2A axis in insect fat body is activated by 20-hydroxyecdysone to antagonize insulin/IGF signaling and restrict growth rate
Author(s) -
DongWei Yuan,
Shun Zhou,
Suning Liu,
Kang Li,
Haigang Zhao,
Shihui Long,
Hanhan Liu,
Yongfang Xie,
YunLin Su,
Fengwei Yu,
Sheng Li
Publication year - 2020
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2000963117
Subject(s) - biology , insulin , ampk , endocrinology , fat body , phosphatase , insulin like growth factor , medicine , protein phosphatase 2 , signal transduction , 20 hydroxyecdysone , microbiology and biotechnology , growth factor , gene , hormone , biochemistry , phosphorylation , receptor , protein kinase a
Significance Final body size determination in animals remains a long-standing puzzle in the field of developmental biology. In insects, insulin/insulin-like growth factor signaling (IIS) and the steroid hormone 20-hydroxyecdysone (20E) are mainly responsible for the growth rate and growth period, respectively, and consequently the final body size. Here, we report that 20E activates AMPK by up-regulating its gene expression and inducing sugar starvation. In turn, AMPK activates protein phosphatase 2A, which further dephosphorylates and inactivates key components in IIS in the fat body, and eventually suppresses the larval growth rate. These data answer the question of how 20E antagonizes IIS and modulates growth rate.
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