Transcriptome profiling reveals signaling conditions dictating human spermatogonia fate in vitro | Zendy

Kun Tan | Zendy; H. C. Song | Zendy; Merlin Thompson | Zendy; Sarah Munyoki | Zendy; Meena Sukhwani | Zendy; TungChin Hsieh | Zendy; Kyle E. Orwig | Zendy; Miles Wilkinson | Zendy

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Transcriptome profiling reveals signaling conditions dictating human spermatogonia fate in vitro

Author(s) -

Kun Tan,

H. C. Song,

Merlin Thompson,

Sarah Munyoki,

Meena Sukhwani,

TungChin Hsieh,

Kyle E. Orwig,

Miles Wilkinson

Publication year - 2020

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.2000362117

Subject(s) - transcriptome , profiling (computer programming) , computational biology , in vitro , biology , microbiology and biotechnology , gene expression profiling , genetics , computer science , gene , gene expression , operating system

Significance The ability to culture human spermatogonial stem cells (SSCs) in vitro is critical to develop SSC therapeutic approaches to treat male infertility. To achieve this goal, it is important to define molecular pathways in human SSCs. Toward this end, we developed an approach to purify human primitive undifferentiated spermatogonia (uSPG) highly enriched in SSCs. Comparative RNA-sequencing analysis of these primitive uSPG versus differentiating SPG identified differentially expressed genes encoding key components in several signaling pathways. Leveraging this information, coupled with single-cell RNA-sequencing analysis, we studied the effects of modulating signaling pathways on human SPG fate in vitro. Inhibition of AKT signaling favored primitive uSPG fate, which can be applied to culture SSCs for therapeutic applications.

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