The biochemical basis for the cooperative action of microRNAs | Zendy

Daniel Briskin | Zendy; Peter Y. Wang | Zendy; David P. Bartel | Zendy

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The biochemical basis for the cooperative action of microRNAs

Author(s) -

Daniel Briskin,

Peter Y. Wang,

David P. Bartel

Publication year - 2020

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.1920404117

Subject(s) - argonaute , psychological repression , microrna , biology , messenger rna , computational biology , function (biology) , rna , microbiology and biotechnology , rna binding protein , genetics , rna interference , gene expression , gene

Significance MicroRNAs (miRNAs) are short RNAs that guide the repression of most human messenger RNAs (mRNAs) and play many important roles in physiology and development. To function, each miRNA associates with an Argonaute (AGO) protein to form a complex in which the miRNA pairs to sites in mRNAs, thereby targeting these mRNAs for repression, and AGO associates with TNRC6, a protein that recruits the mRNA-repression machinery. For previously unknown reasons, repression is often more effective when miRNAs pair to sites close to one another. We find that in the presence of the TNRC6 AGO-binding domain, which can simultaneously bind multiple AGO proteins, miRNA-AGO complexes bind a two-site target RNA with high cooperatively, providing a biochemical explanation for cooperative action in cells.

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