Open AccessAn in vivo RNAi screen uncovers the role of AdoR signaling and adenosine deaminase in controlling intestinal stem cell activity
Author(s) -
Chiwei Xu,
Brian Franklin,
Hong-Wen Tang,
Yannik RégimbaldDumas,
Yanhui Hu,
Justine Ramos,
Justin A. Bosch,
Christians Villalta,
Xi He,
Norbert Perrimon
Publication year - 2019
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.1900103117
Subject(s) - enterocyte , adenosine , microbiology and biotechnology , stem cell , adenosine deaminase , purinergic signalling , biology , purinergic receptor , in vivo , signal transduction , intestinal epithelium , adenosine receptor , extracellular , receptor , epithelium , small intestine , biochemistry , genetics , agonist
Significance Regulation of stem cells by microenvironment signals is important to maintain epithelial homeostasis. Using a quantitative readout, we screened for receptor genes that affect the intestinal stem cell pool size in the adultDrosophila midgut. The top candidate of our screen,AdoR , underscores the importance of purinergic signaling in controlling ISC activity. Furthermore, we identified a pivotal role of an enterocyte-derived metabolic enzyme, Adgf-A, in limiting the activity of extracellular adenosine and shaping the ISC microenvironment.
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