Open AccessTIM-mediated inhibition of HIV-1 release is antagonized by Nef but potentiated by SERINC proteins
Author(s) -
Minghua Li,
Abdül Waheed,
Jingyou Yu,
Cong Zeng,
Hui-Yu Chen,
Yi-Min Zheng,
Amin Feizpour,
Björn M. Reinhard,
Suryaram Gummuluru,
Steven Lin,
Eric O. Freed,
ShanLu Liu
Publication year - 2019
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.1819475116
Subject(s) - human immunodeficiency virus (hiv) , microbiology and biotechnology , virology , function (biology) , biology , ectopic expression , chemistry , virus , cell culture , genetics
Significance TIM proteins inhibit release of HIV-1 and other enveloped viruses. However, it is currently unknown whether and how the virus counteracts this restriction. In this work, we demonstrate that Nef proteins of HIV-1 and other lentiviruses function as antagonists to overcome the TIM-mediated restriction. TIM-1 is more potent at inhibiting release of Nef-deficient HIV-1 relative to wild-type (WT) HIV-1, and ectopic expression of Nef relieves this restriction. Interestingly, we find that SERINC proteins potentiate TIM-mediated inhibition of HIV-1 release likely by stabilizing TIM-1 expression. Our work reveals a role for lentiviralNef in antagonizing TIMs, in part through SERINCs.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom