Open AccessAnaphase-promoting complex/cyclosome regulates RdDM activity by degrading DMS3 in Arabidopsis
Author(s) -
Songxiao Zhong,
Yi-Feng Xu,
Chaoyi Yu,
Xiaotuo Zhang,
Lei Li,
Haoran Ge,
Guodong Ren,
Yingxiang Wang,
Jinbiao Ma,
Yun Zheng,
Binglian Zheng
Publication year - 2019
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.1816652116
Subject(s) - biology , arabidopsis , microbiology and biotechnology , dna methylation , mutant , ubiquitin , cell cycle , genetics , gene , gene expression
Significance RNA-directed DNA methylation (RdDM) is an important mechanism in the silencing of transposable elements in plants. The framework of RdDM has been well established, in which DNA polymerase IV (Pol IV) and Pol V act in upstream siRNA biogenesis and downstream DNA methylation, respectively. The DDR complex, consisting of DMS3, DRD1, and RDM1, is responsible for recruiting Pol V, and dysfunction of any of them causes compromised RdDM activity. However, how the association among DMS3, DRD1, and RDM1 is regulated is unexplored. We show here that the anaphase-promoting complex/cyclosome (APC/C), a key cell cycle regulatory complex, regulates the assembly of the DDR complex by targeting DMS3 for degradation, and thereby provide a perspective in understanding the regulatory mechanism of RdDM activity.
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