Open AccessIn vivoimmunological function of mast cells derived from embryonic stem cells: An approach for the rapid analysis of even embryonic lethal mutations in adult micein vivo
Author(s) -
Mindy Tsai,
Jochen Wedemeyer,
Soula Ganiatsas,
SeeYing Tam,
Leonard I. Zon,
Stephen J. Galli
Publication year - 2000
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.160254997
Subject(s) - embryonic stem cell , biology , stem cell , microbiology and biotechnology , transplantation , in vivo , in vitro , mast cell , immunology , genetics , gene , medicine , surgery
An important goal of tissue engineering is to achieve reconstitution of specific functionally active cell types by transplantation of differentiated cell populations derived from normal or genetically altered embryonic stem cellsin vitro . We find that mast cells derivedin vitro from wild-type or genetically manipulated embryonic stem cells can survive and orchestrate immunologically specific IgE-dependent reactions after transplantation into mast cell-deficientKit W /Kit W-v mice. These findings define a unique approach for analyzing the effects of mutations of any genes that are expressed in mast cells, including embryonic lethal mutations,in vitro orin vivo .