Open AccessInfluence of the period -dependent circadian clock on diurnal, circadian, and aperiodic gene expression in Drosophila melanogaster
Author(s) -
Yiing Lin,
Mei Han,
Brian Shimada,
Lin Wang,
Therese M. Gibler,
Aloka Amarakone,
Tarif Awad,
Gary D. Stormo,
Russell N. Van Gelder,
Paul H. Taghert
Publication year - 2002
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.132269699
Subject(s) - circadian rhythm , period (music) , biology , drosophila melanogaster , gene , gene expression , timeless , circadian clock , darkness , genetics , mutant , clock , oscillating gene , microbiology and biotechnology , endocrinology , botany , physics , acoustics
We measured daily gene expression in heads of control and period mutant Drosophila by using oligonucleotide microarrays. In control flies, 72 genes showed diurnal rhythms in light-dark cycles; 22 of these also oscillated in free-running conditions. The period gene significantly influenced the expression levels of over 600 nonoscillating transcripts. Expression levels of several hundred genes also differed significantly between control flies kept in light-dark versus constant darkness but differed minimally between per(01) flies kept in the same two conditions. Thus, the period-dependent circadian clock regulates only a limited set of rhythmically expressed transcripts. Unexpectedly, period regulates basal and light-regulated gene expression to a very broad extent.