Rad54 protein stimulates the postsynaptic phase of Rad51 protein-mediated DNA strand exchange | Zendy

Jachen A. Solinger | Zendy; WolfDietrich Heyer | Zendy

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Rad54 protein stimulates the postsynaptic phase of Rad51 protein-mediated DNA strand exchange

Author(s) -

Jachen A. Solinger,

WolfDietrich Heyer

Publication year - 2001

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.121009898

Subject(s) - rad51 , replication protein a , ddb1 , microbiology and biotechnology , biology , dna , dna repair , chemistry , biochemistry , dna binding protein , gene , transcription factor

Rad54 and Rad51 are important proteins for the repair of double-stranded DNA breaks by homologous recombination in eukaryotes. As previously shown, Rad51 protein forms nucleoprotein filaments on single-stranded DNA, and Rad54 protein directly interacts with such filaments to enhance synapsis, the homologous pairing with a double-stranded DNA partner. Here we demonstrate that Saccharomyces cerevisiae Rad54 protein has an additional role in the postsynaptic phase of DNA strand exchange by stimulating heteroduplex DNA extension of established joint molecules in Rad51/Rpa-mediated DNA strand exchange. This function depended on the ATPase activity of Rad54 protein and on specific protein:protein interactions between the yeast Rad54 and Rad51 proteins.

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