Open AccessReduced production of B-1–specified common lymphoid progenitors results in diminished potential of adult marrow to generate B-1 cells
Author(s) -
Chad L. Barber,
Encarnación Montecino-Rodriguez,
Kenneth Dorshkind
Publication year - 2011
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.1107172108
Subject(s) - lymphopoiesis , progenitor cell , haematopoiesis , biology , stem cell , progenitor , b cell , compartment (ship) , immunology , in vivo , microbiology and biotechnology , genetics , antibody , oceanography , geology
B-1 B cells have been proposed to be preferentially generated from fetal progenitors, but this view is challenged by studies concluding that B-1 production is sustained throughout adult life. To address this controversy, we compared the efficiency with which hematopoietic stem cells (HSCs) and common lymphoid progenitors (CLPs) from neonates and adults generated B-1 cells in vivo and developed a clonal in vitro assay to quantify B-1 progenitor production from CLPs. Adult HSCs and CLPs generated fewer B-1 cells in vivo compared with their neonatal counterparts, a finding corroborated by the clonal studies that showed that the CLP compartment includes B-1– and B-2–specified subpopulations and that the former cells decrease in number after birth. Together, these data indicate that B-1 lymphopoiesis is not sustained at constant levels throughout life and define a heretofore unappreciated developmental heterogeneity within the CLP compartment.