Open AccessMolecular distinction in genetic regulation of nonphotochemical quenching in rice
Author(s) -
Ichiro Kasajima,
Kaworu Ebana,
Tetsuro Yamamoto,
Kentaro Takahara,
Masahiro Yano,
Maki KawaiYamada,
Hirofumi Uchimiya
Publication year - 2011
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.1104809108
Subject(s) - cultivar , japonica , biology , non photochemical quenching , mutant , photosynthesis , oryza sativa , gene , genetically modified rice , botany , photosystem ii , photoinhibition , transgene , genetically modified crops , genetics
Nonphotochemical quenching (NPQ) regulates energy conversion in photosystem II and protects plants from photoinhibition. Here we analyze NPQ capacity in a number of rice cultivars. NPQ was strongly induced under medium and high light intensities in rice leaves. Japonica cultivars generally showed higher NPQ capacities than Indica cultivars when we measured a rice core collection. We mapped NPQ regulator and identified a locus (qNPQ1-2 ) that seems to be responsible for the difference in NPQ capacity between Indica and Japonica. One of the two ricePsbS homologues (OsPsbS1 ) was found within theqNPQ1-2 region. PsbS protein was not accumulated in the leaf blade of the mutant harboring transferred DNA insertion inOsPsbS1 . NPQ capacity increased asOsPsbS1 expression increased in a series of transgenic lines ectopically expressingOsPsbS1 in an Indica cultivar. Indica cultivars lack a 2.7-kb region at the point 0.4 kb upstream of theOsPsbS1 gene, suggesting evolutionary discrimination of this gene.