Open AccessMutated β-catenin evades a microRNA-dependent regulatory loop
Author(s) -
Angelo Veronese,
Rosa Visone,
Jessica Consiglio,
Mario Acunzo,
Laura Lupini,
Taewan Kim,
Manuela Ferracin,
Francesca Lovat,
Elena Miotto,
Veronica Balatti,
Lucilla D’Abundo,
Laura Gramantieri,
Luigi Bolondi,
Yuri Pekarsky,
Danilo Perrotti,
Massimo Negrini,
Carlo M. Croce
Publication year - 2011
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.1101734108
Subject(s) - puma , biology , microrna , transcription factor , gene , genetics , regulation of gene expression , ectopic expression , microbiology and biotechnology
hsa-mir-483 is located within intron 2 of the IGF2 gene. We have previously shown oncogenic features of miR-483-3p through cooperation with IGF2 or by independently targeting the proapoptotic gene BBC3/PUMA. Here we demonstrate that expression of miR-483 can be induced independently of IGF2 by the oncoprotein β-catenin through an interaction with the basic helix-loop-helix protein upstream stimulatory transcription factor 1. We also show that β-catenin itself is a target of miR-483-3p, triggering a negative regulatory loop that becomes ineffective in cells harboring an activating mutation of β-catenin. These results provide insights into the complex regulation of the IGF2/miR-483 locus, revealing players in the β-catenin pathway.