Open AccessRegulation of induced colonic inflammation byLactobacillus acidophilusdeficient in lipoteichoic acid
Author(s) -
Mansour Mohamadzadeh,
Erika A. Pfeiler,
Jeffrey B. Brown,
Matthew Gramarossa,
Elizabeth Managlia,
Praveen Bere,
Bara Sarraj,
Mohammad W. Khan,
Krishna Chaitanya Pakanati,
Mohammed Javeed Ansari,
Sarah O’Flaherty,
Terrence Barrett,
Todd R. Klaenhammer
Publication year - 2011
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.1005066107
Subject(s) - lipoteichoic acid , lactobacillus acidophilus , proinflammatory cytokine , colitis , inflammation , immune system , immunology , biology , intestinal mucosa , inflammatory bowel disease , foxp3 , medicine , probiotic , disease , bacteria , genetics , staphylococcus aureus
Imbalance in the regulatory immune mechanisms that control intestinal cellular and bacterial homeostasis may lead to induction of the detrimental inflammatory signals characterized in humans as inflammatory bowel disease. Induction of proinflammatory cytokines (i.e., IL-12) induced by dendritic cells (DCs) expressing pattern recognition receptors may skew naive T cells to T helper 1 polarization, which is strongly implicated in mucosal autoimmunity. Recent studies show the ability of probiotic microbes to treat and prevent numerous intestinal disorders, includingClostridium difficile -induced colitis. To study the molecular mechanisms involved in the induction and repression of intestinal inflammation, the phosphoglycerol transferase gene that plays a key role in lipoteichoic acid (LTA) biosynthesis inLactobacillus acidophilus NCFM (NCK56) was deleted. The data show that theL. acidophilus LTA-negative in LTA (NCK2025) not only down-regulated IL-12 and TNFα but also significantly enhanced IL-10 in DCs and controlled the regulation of costimulatory DC functions, resulting in their inability to induce CD4+ T-cell activation. Moreover, treatment of mice with NCK2025 compared with NCK56 significantly mitigated dextran sulfate sodium and CD4+ CD45RBhigh T cell-induced colitis and effectively ameliorated dextran sulfate sodium-established colitis through a mechanism that involves IL-10 and CD4+ FoxP3+ T regulatory cells to dampen exaggerated mucosal inflammation. Directed alteration of cell surface components ofL. acidophilus NCFM establishes a potential strategy for the treatment of inflammatory intestinal disorders.