His-tags lighten up and lose their inhibitions | Zendy

Michael C. Pirrung | Zendy

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His-tags lighten up and lose their inhibitions

Author(s) -

Michael C. Pirrung

Publication year - 2010

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.1002011107

Subject(s) - longevity , history , demography , gerontology , genealogy , medicine , sociology

Cartoon of the light-directed immobilization of a His-tagged protein on a microarray using the autoinhibition method. An undecapeptide construct (green) is created with a nickel-tris-(nitrilotriacetic acid) multivalent head group (nickel-tris-NTA) at the N terminus and a His-tag at the C terminus. Included within the peptide sequence is the photocleavable amino acid 3-amino-3-(2-nitrophenyl)-propionic acid and a single Cys for attachment of the construct to a microarray (or other molecules, in further …

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