Open AccessNickel-inducible lysis system in Synechocystis sp. PCC 6803
Author(s) -
Xinyao Liu,
Roy Curtiss
Publication year - 2009
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0911953106
Subject(s) - lysis , synechocystis , bacteriophage , lysin , cyanobacteria , biology , gene , lysogenic cycle , autolysis (biology) , biochemistry , chemistry , microbiology and biotechnology , enzyme , bacteria , genetics , mutant , escherichia coli
We designed and constructed a controllable inducing lysis system inSynechocystis sp. PCC 6803 to facilitate extracting lipids for biofuel production. Several bacteriophage-derived lysis genes were integrated into the genome and placed downstream of a nickel-inducible signal transduction system. We applied 3 strategies: (i ) directly using the phage lysis cassette, (ii ) constitutively expressing endolysin genes while restricting holin genes, and (iii ) combining lysis genes from different phages. Significant autolysis was induced in theSynechocystis sp. PCC 6803 cells with this system by the addition of NiSO4 . Our inducible cyanobacterial lysing system eliminates the need for mechanical or chemical cell breakage and could facilitate recovery of biofuel from cyanobacteria.
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