Electron microscopy of whole cells in liquid with nanometer resolution | Zendy

Niels de Jonge | Zendy; Diana B. Peckys | Zendy; GertJan Kremers | Zendy; David W. Piston | Zendy

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Electron microscopy of whole cells in liquid with nanometer resolution

Author(s) -

Niels de Jonge,

Diana B. Peckys,

GertJan Kremers,

David W. Piston

Publication year - 2009

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.0809567106

Subject(s) - resolution (logic) , electron microscope , transmission electron microscopy , microscope , materials science , scanning transmission electron microscopy , conventional transmission electron microscope , microscopy , dwell time , scanning electron microscope , nanotechnology , optics , chemistry , physics , medicine , clinical psychology , artificial intelligence , computer science , composite material

Single gold-tagged epidermal growth factor (EGF) molecules bound to cellular EGF receptors of fixed fibroblast cells were imaged in liquid with a scanning transmission electron microscope (STEM). The cells were placed in buffer solution in a microfluidic device with electron transparent windows inside the vacuum of the electron microscope. A spatial resolution of 4 nm and a pixel dwell time of 20 micros were obtained. The liquid layer was sufficiently thick to contain the cells with a thickness of 7 +/- 1 microm. The experimental findings are consistent with a theoretical calculation. Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods.

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