MicroRNA-directed transcriptional gene silencing in mammalian cells | Zendy

Daniel H. Kim | Zendy; Pål Sætrom | Zendy; Ola Snøve | Zendy; John J. Rossi | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

MicroRNA-directed transcriptional gene silencing in mammalian cells

Author(s) -

Daniel H. Kim,

Pål Sætrom,

Ola Snøve,

John J. Rossi

Publication year - 2008

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.0808830105

Subject(s) - biology , argonaute , gene silencing , rna induced silencing complex , rna interference , microrna , regulation of gene expression , epigenetics , genetics , gene expression , gene , trans acting sirna , polycomb group proteins , microbiology and biotechnology , rna , repressor

MicroRNAs (miRNAs) regulate gene expression at the posttranscriptional level in the cytoplasm, but recent findings suggest additional roles for miRNAs in the nucleus. To address whether miRNAs might transcriptionally silence gene expression, we searched for miRNA target sites proximal to known gene transcription start sites in the human genome. One conserved miRNA, miR-320, is encoded within the promoter region of the cell cycle gene POLR3D in the antisense orientation. We provide evidence of a cis-regulatory role for miR-320 in transcriptional silencing of POLR3D expression. miR-320 directs the association of RNA interference (RNAi) protein Argonaute-1 (AGO1), Polycomb group (PcG) component EZH2, and tri-methyl histone H3 lysine 27 (H3K27me3) with the POLR3D promoter. Our results suggest the existence of an epigenetic mechanism of miRNA-directed transcriptional gene silencing (TGS) in mammalian cells.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research