Open AccessHigh field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents
Author(s) -
Boris Polyak,
Ilia Fishbein,
Michael Chorny,
Ivan S. Alferiev,
Darryl Williams,
Ben Yellen,
Gary D. Friedman,
Robert J. Levy
Publication year - 2008
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0708338105
Subject(s) - in vivo , materials science , stent , magnetic field , nanotechnology , superparamagnetism , biomedical engineering , magnetic nanoparticles , luciferase , in vitro , nanoparticle , biophysics , chemistry , cell culture , biology , medicine , radiology , biochemistry , magnetization , transfection , physics , microbiology and biotechnology , genetics , quantum mechanics
A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i ) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii ) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires.In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires.In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected byin vivo optical imaging, than nonmagnetic controls.