A regulatory protein that interferes with activator-stimulated transcription in bacteria | Zendy

Shunji Nakano | Zendy; Michiko Nakano | Zendy; Ying Zhang | Zendy; Montira Leelakriangsak | Zendy; Peter Zuber | Zendy

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A regulatory protein that interferes with activator-stimulated transcription in bacteria

Author(s) -

Shunji Nakano,

Michiko Nakano,

Ying Zhang,

Montira Leelakriangsak,

Peter Zuber

Publication year - 2003

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.0637648100

Subject(s) - rna polymerase , bacterial transcription , activator (genetics) , transcription (linguistics) , biology , bacillus subtilis , protein subunit , microbiology and biotechnology , dna binding protein , transcription factor , promoter , transcriptional regulation , sigma factor , biochemistry , rna , bacteria , genetics , gene , gene expression , linguistics , philosophy

Transcriptional activator proteins in bacteria often operate by interaction with the C-terminal domain of the alpha-subunit of RNA polymerase (RNAP). Here we report the discovery of an "anti-alpha" factor Spx in Bacillus subtilis that blocks transcriptional activation by binding to the alpha-C-terminal domain, thereby interfering with the capacity of RNAP to respond to certain activator proteins. Spx disrupts complex formation between the activator proteins ResD and ComA and promoter-bound RNAP, and it does so by direct interaction with the alpha-subunit. ResD- and ComA-stimulated transcription requires the proteolytic elimination of Spx by the ATP-dependent protease ClpXP. Spx represents a class of transcriptional regulators that inhibit activator-stimulated transcription by interaction with alpha.

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