Inducible protein knockout reveals temporal requirement of CaMKII reactivation for memory consolidation in the brain | Zendy

Huimin Wang | Zendy; Eiji Shimizu | Zendy; YaPing Tang | Zendy; Min Cho | Zendy; Maureen Kyin | Zendy; Wenqi Zuo | Zendy; D Robinson | Zendy; Peter J. Alaimo | Zendy; Chao Zhang | Zendy; Hiromi Morimoto | Zendy; Min Zhuo | Zendy; Ruiben Feng | Zendy; Kevan M. Shokat | Zendy; Joe Z. Tsien | Zendy

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Inducible protein knockout reveals temporal requirement of CaMKII reactivation for memory consolidation in the brain

Author(s) -

Huimin Wang,

Eiji Shimizu,

YaPing Tang,

Min Cho,

Maureen Kyin,

Wenqi Zuo,

D Robinson,

Peter J. Alaimo,

Chao Zhang,

Hiromi Morimoto,

Min Zhuo,

Ruiben Feng,

Kevan M. Shokat,

Joe Z. Tsien

Publication year - 2003

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.0636870100

Subject(s) - knockout mouse , consolidation (business) , memory consolidation , conditional gene knockout , calmodulin , neuroscience , microbiology and biotechnology , in vivo , biology , protein engineering , phenotype , genetics , receptor , biochemistry , gene , enzyme , accounting , hippocampus , business

By integrating convergent protein engineering and rational inhibitor design, we have developed an in vivo conditional protein knockout andor manipulation technology. This method is based on the creation of a specific interaction interface between a modified protein domain and sensitized inhibitors. By introducing this system into genetically modified mice, we can readily manipulate the activity of a targeted protein, such as alpha-Ca(2+)calmodulin-dependent protein kinase II (alphaCAMKII), on the time scale of minutes in specific brain subregions of freely behaving mice. With this inducible and region-specific protein knockout technique, we analyzed the temporal stages of memory consolidation process and revealed the first postlearning week as the critical time window during which a precise level of CaMKII reactivation is essential for the consolidation of long-term memories in the brain.

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