Open AccessCoadministration of HIV vaccine vectors with vaccinia viruses expressing IL-15 but not IL-2 induces long-lasting cellular immunity
Author(s) -
Sang Kon Oh,
Jay A. Berzofsky,
Donald S. Burke,
Thomas A. Waldmann,
Liyanage P. Perera
Publication year - 2003
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0630592100
Subject(s) - vaccinia , immunity , biology , cellular immunity , cytotoxic t cell , priming (agriculture) , virology , immunology , cd8 , hiv vaccine , cytokine , immune system , interleukin 15 , antibody , humoral immunity , interleukin , vaccine trial , in vitro , recombinant dna , biochemistry , botany , germination , gene
Vaccine efficacy is determined largely by cellular and humoral immunity as well as long-lasting immunological memory. IL-2 and IL-15 were evaluated in vaccinia vectors expressing HIV gp160 for the establishment of an effective vaccine strategy. Both IL-2 and IL-15 in the vaccinia vector induced strong and long-lasting antibody-mediated immunity as well as a short-term cytotoxic T cell response against HIV gp120. In addition, IL-15 also supported robust CD8+ T cell-mediated long-term immunity, whereas the CD8+ T cell-mediated immunity induced by IL-2 was short-lived. Moreover, we found that the cytokine milieu at the time of priming had surprisingly persistent effects on the character of the memory CD8 T cells long afterward with respect to their fate, functional activities, cytokine receptor expression, and antigen-independent proliferation.