Open AccessNMDA receptor function is regulated by the inhibitory scaffolding protein, RACK1
Author(s) -
Rami Yaka,
Claire Thornton,
Alicia Vagts,
Khanhky Phamluong,
Antonello Bonci,
Dorit Ron
Publication year - 2002
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.062046299
Subject(s) - fyn , nmda receptor , phosphorylation , microbiology and biotechnology , scaffold protein , chemistry , tyrosine phosphorylation , receptor , biochemistry , biology , signal transduction , proto oncogene tyrosine protein kinase src
Phosphorylation regulates the function of ligand-gated ion channels such as the N-methyl d-aspartate (NMDA) receptor. Here we report a mechanism for modulation of the phosphorylation state and function of the NMDA receptor via an inhibitory scaffolding protein, RACK1. We found that RACK1 binds both the NR2B subunit of the NMDA receptor and the nonreceptor protein tyrosine kinase, Fyn. RACK1 inhibits Fyn phosphorylation of NR2B and decreases NMDA receptor-mediated currents in CA1 hippocampal slices. Peptides that disrupt the interactions between RACK1, NR2B, and Fyn induce phosphorylation and potentiate NMDA receptor-mediated currents. Therefore, RACK1 is a regulator of NMDA receptor function and may play a role in synaptic plasticity, addiction, learning, and memory.
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