Open AccessAssisted reproductive technologies do not alter mutation frequency or spectrum
Author(s) -
Lee Caperton,
Patricia Murphey,
Yukiko Yamazaki,
C. Alex McMahan,
Christi A. Walter,
Ryuzo Yanagimachi,
John R. McCarrey
Publication year - 2007
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0611642104
Subject(s) - intracytoplasmic sperm injection , spermatid , biology , reproductive technology , in vitro fertilisation , andrology , embryo , sperm , human fertilization , embryo transfer , genetics , transgene , embryogenesis , medicine , gene
Assisted reproductive technologies (ARTs) have now contributed to the birth of >3 million babies worldwide, but concerns remain regarding the safety of these methods. We have used a transgenic mouse model to examine the effects of ARTs on the frequency and spectrum of point mutations in midgestation mouse fetuses produced by either natural reproduction or various methods of ART, including preimplantation culture, embryo transfer,in vitro fertilization, intracytoplasmic sperm injection, and round spermatid injection. Our results show that there is no significant difference in the frequency or spectrum ofde novo point mutations found in naturally conceived fetuses and fetuses produced byin vitro fertilization, intracytoplasmic sperm injection, or round spermatid injection. These results, based on analyses of a transgenic mouse system, indicate that with respect to maintenance of genetic integrity, ARTs appear to be safe.