Open AccessRNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing
Author(s) -
David A. Schneider,
Sarah L. French,
Yvonne N. Osheim,
Aaron O. Bailey,
L Vu,
Jonathan A. Dodd,
John R. Yates,
Ann L. Beyer,
Michio Nomura
Publication year - 2006
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0605686103
Subject(s) - rna polymerase ii , transcription (linguistics) , biology , transcription factor ii d , rna polymerase ii holoenzyme , rna polymerase i , transcription factor ii f , rna polymerase iii , ribosomal rna , general transcription factor , rna polymerase , termination factor , microbiology and biotechnology , transcription factor ii e , polymerase , ribosome , rna , genetics , gene , promoter , gene expression , linguistics , philosophy
Previous investigations into the mechanisms that control RNA Polymerase (Pol) I transcription have primarily focused on the process of transcription initiation, thus little is known regarding postinitiation steps in the transcription cycle. Spt4p and Spt5p are conserved throughout eukaryotes, and they affect elongation by Pol II. We have found that these two proteins copurify with Pol I and associate with the rDNA in vivo. Disruption of the gene for Spt4p resulted in a modest decrease in growth and rRNA synthesis rates at the permissive temperature, 30 degrees C. Furthermore, biochemical and EM analyses showed clear defects in rRNA processing. These data suggest that Spt4p, Spt5p, and, potentially, other regulators of Pol I transcription elongation play important roles in coupling rRNA transcription to its processing and ribosome assembly.
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