Open AccessA genomewide screen for components of the RNAi pathway in Drosophila cultured cells
Author(s) -
Silke Dorner,
Lawrence Lum,
Michelle Kim,
Renato Paro,
Philip A. Beachy,
Rachel Green
Publication year - 2006
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0605210103
Subject(s) - rna interference , drosophila melanogaster , biology , gene silencing , gene , genetics , rna silencing , small interfering rna , function (biology) , microbiology and biotechnology , luciferase , rna , transfection
Posttranscriptional silencing by RNAi is initiated by dsRNAs that are processed into siRNAs that ultimately target homologous mRNAs for degradation. We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway inDrosophila melanogaster . The screen identified seven genes that affect the RNAi response, five with previously described function (AGO2 ,Tis11 ,Hsc70-3 ,Hsc70-4 , andhdc ) and two annotated genes (CG17265 andCG10883 ).