Open AccessOrdered assembly of the duplicating Golgi in Trypanosoma brucei
Author(s) -
Helen H. Ho,
Cynthia Y. He,
Christopher L. de Graffenried,
Lindsay J. Murrells,
Graham Warren
Publication year - 2006
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0602595103
Subject(s) - golgi apparatus , endoplasmic reticulum , trypanosoma brucei , microbiology and biotechnology , copi , biology , secretory pathway , trypanosoma , biochemistry , genetics , gene
The new Golgi in the protozoan parasiteTrypanosoma brucei grows near to the old and adjacent to the growing new endoplasmic reticulum exit site. Growth is now shown to be at least a two-stage process, in which a representative matrix marker (GRASP) and enzyme (GntB) are delivered to the site of assembly, followed ≈10 min later by a COPI component (ε-COP) and a trans-Golgi network (TGN) marker (GRIP70). A secretory cargo marker (signal sequence-YFP) appeared early near the new endoplasmic reticulum exit site but did not enter the Golgi until the second stage. Together these data suggest that structural and enzymatic components of the new Golgi stack are laid down first, followed by those needed to move and sort the cargo passing through it.