Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis | Zendy

David Drew | Zendy; Dan Sjöstrand | Zendy; Johan Nilsson | Zendy; Thomas Urbig | Zendy; ChenNi Chin | Zendy; JanWillem De Gier | Zendy; Gunnar von Heijne | Zendy

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Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis

Author(s) -

David Drew,

Dan Sjöstrand,

Johan Nilsson,

Thomas Urbig,

ChenNi Chin,

JanWillem De Gier,

Gunnar von Heijne

Publication year - 2002

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.052018199

Subject(s) - escherichia coli , green fluorescent protein , fusion , fusion protein , membrane protein , inner membrane , microbiology and biotechnology , topology (electrical circuits) , chemistry , membrane , biology , computational biology , biochemistry , gene , recombinant dna , engineering , philosophy , linguistics , electrical engineering

We present an approach that allows rapid determination of the topology of Escherichia coli inner-membrane proteins by a combination of topology prediction and limited fusion-protein analysis. We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA. We estimate that our approach should make it possible to arrive at highly reliable topology models for roughly 10% of the approximately 800 inner-membrane proteins thought to exist in E. coli.

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