Open AccessRequirement of myocardin-related transcription factor-B for remodeling of branchial arch arteries and smooth muscle differentiation
Author(s) -
Jiyeon Oh,
James A. Richardson,
Eric N. Olson
Publication year - 2005
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0507346102
Subject(s) - myocardin , biology , serum response factor , neural crest , transcription factor , branchial arch , microbiology and biotechnology , endocrinology , anatomy , medicine , gene , genetics , embryo
Myocardin and the myocardin-related transcription factors (MRTFs) A and B act as coactivators for serum response factor, which plays a key role in cardiovascular development. To determine the functions of MRTF-Bin vivo , we generatedMRTF-B mutant mice by targeted inactivation of theMRTF-B gene. We show that mice homozygous for anMRTF-B loss-of-function mutation die during mid-gestation from a spectrum of cardiovascular defects that includes abnormal patterning of the branchial arch arteries, double-outlet right ventricle, ventricular septal defects, and thin-walled myocardium. These abnormalities are accompanied by a failure in differentiation of smooth muscle cells within the branchial arch arteries, which are derived from the neural crest. The phenotype ofMRTF-B mutant mice is distinct from that of mice lacking myocardin, revealing unique roles for these serum response factor coactivators in the development of different subsets of smooth muscle cellsin vivo .