Mutated plant lectin library useful to identify different cells | Zendy

Mijung Yim | Zendy; Takashi Ono | Zendy; Tatsuro Irimura | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Mutated plant lectin library useful to identify different cells

Author(s) -

Mijung Yim,

Takashi Ono,

Tatsuro Irimura

Publication year - 2001

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.041621998

Subject(s) - lectin , c type lectin , mutant , biology , cdna library , biochemistry , recombinant dna , polyclonal antibodies , escherichia coli , agglutination (biology) , hemagglutinin (influenza) , complementary dna , mutagenesis , fusion protein , microbiology and biotechnology , antibody , gene , genetics

The 24 nucleotides comprising the carbohydrate-recognition domain of Maackia amurensis hemagglutinin (MAH) cDNA were randomly mutated. The mutant lectins were expressed as glutathione-S-transferase fusion proteins in Escherichia coli and 16 clones were randomly chosen. Although all of 16 recombinant lectins reacted strongly with anti-MAH polyclonal antibody, the carbohydrate-recognition domain of each was unique. As shown by agglutination studies, each mutant MAH lectin was able to bind to erythrocytes from one or more of five animal species in very distinct patterns. Thus, novel plant lectin libraries can be used to discriminate in a highly specific manner among a variety of cell types. This technology may prove to be very useful in a number of different applications requiring a high level of specificity in cell identification.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research