Inositol 1,4,5-trisphosphate receptor/GAPDH complex augments Ca 2+ release via locally derived NADH | Zendy

Randen L. Patterson | Zendy; Damian B. van Rossum | Zendy; Adam Kaplin | Zendy; Roxanne K. Barrow | Zendy; Solomon H. Snyder | Zendy

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Inositol 1,4,5-trisphosphate receptor/GAPDH complex augments Ca ²⁺ release via locally derived NADH

Author(s) -

Randen L. Patterson,

Damian B. van Rossum,

Adam Kaplin,

Roxanne K. Barrow,

Solomon H. Snyder

Publication year - 2005

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.0409657102

Subject(s) - glyceraldehyde 3 phosphate dehydrogenase , inositol , inositol trisphosphate receptor , endoplasmic reticulum , calcium , nad+ kinase , inositol trisphosphate , calcium signaling , intracellular , calcium in biology , receptor , biochemistry , chemistry , microbiology and biotechnology , biology , enzyme , dehydrogenase , organic chemistry

NADH regulates the release of calcium from the endoplasmic reticulum by modulation of inositol 1,4,5-trisphosphate receptors (IP3R), accounting for the augmented calcium release of hypoxic cells. We report selective binding of IP3R to GAPDH, whose activity leads to the local generation of NADH to regulate intracellular calcium signaling. This interaction requires cysteines 992 and 995 of IP3R and C150 of GAPDH. Addition of native GAPDH and NAD+ to WT IP3R stimulates calcium release, whereas no stimulation occurs with C992S/995S IP3R that cannot bind GAPDH. Thus, the IP3R/GAPDH interaction likely enables cellular energy dynamics to impact calcium signaling.

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